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Gewählte Publikation:

Uhnakova, B; Ludwig, R; Peknicova, J; Homolka, L; Lisa, L; Sulc, M; Petrickova, A; Elzeinova, F; Pelantova, H; Monti, D; Kren, V; Haltrich, D; Martinkova, L.
(2011): Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products
BIORESOURCE TECHNOL. 2011; 102(20): 9409-9415. FullText FullText_BOKU

Abstract:
Tetrabromobisphenol A (TBBPA) degradation was investigated using white rot fungi and their oxidative enzymes. Strains of the Trametes, Pleurotus, Bjerkandera and Dichomitus genera eliminated almost 1 mM TBBPA within 4 days. Laccase, whose role in TBBPA degradation was demonstrated in fungal cultures, was applied to TBBPA degradation alone and in combination with cellobiose dehydrogenase from Sclerotium rolfsii. Purified laccase from Trametes versicolor degraded approximately 2 mM TBBPA within 5 h, while the addition of cellobiose dehydrogenase increased the degradation rate to almost 2.5 mM within 3 h. Laccase was used to prepare TBBPA metabolites 2,6-dibromo-4-(2-hydroxypropane-2-yl) phenol (1), 2,6-dibromo-4-(2-methoxypropane-2-yl) phenol (2) and 1-(3,5-dibromo-4-hydroxyphen-1-y1)-2,2xxx,6,6xxx-tetrabromo-4,4xxx-isopropylidene diphenol (3). As compounds 1 and 3 were identical to the TBBPA metabolites prepared by using rat and human liver fractions (Zalko et al., 2006), laccase can provide a simple means of preparing these metabolites for toxicity studies. Products 1 and 3 exhibited estrogenic effects, unlike TBBPA, but lower cell toxicity. (C) 2011 Elsevier Ltd. All rights reserved.
Autor/innen der BOKU Wien:
Haltrich Dietmar
Ludwig Roland
BOKU Gendermonitor:


Find related publications in this database (Keywords)
White rot fungi
Laccase
Cellobiose dehydrogenase
Tetrabromobisphenol A metabolites
Estrogenic activity


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