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Gewählte Publikation:

Sygmund, C; Klausberger, M; Felice, AK; Ludwig, R.
(2011): Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity
MICROBIOL-SGM. 2011; 157: 3203-3212. FullText FullText_BOKU

Abstract:
The plant-pathogenic fungus Glomerella cingulata (anamorph Colletotachum gloeosporoides) secretes high levels of an FAD-dependent glucose dehydrogenase (GDH) when grown on tomato juice-supplemented media. To elucidate its molecular and catalytic properties, GDH was produced in submerged culture. The highest volumetric activity was obtained in shaking flasks after 6 days of cultivation (3400 U l(-1), 4.2% of total extracellular protein). GDH is a monomeric protein with an isoelectric point of 5.6. The molecular masses of the glycoforms ranged from 95 to 135 kDa, but after deglycosylation, a single 68 kDa band was obtained. The absorption spectrum is typical for an FAD-containing enzyme with maxima at 370 and 458 nm and the cofactor is non-covalently bound. The preferred substrates are glucose and xylose. Suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. In contrast, oxygen turnover is very low. The GDH-encoding gene was cloned and phylogenetic analysis of the translated protein reveals its affiliation to the GMC family of oxidoreductases. The proposed function of this quinone and phenoxy radical reducing enzyme is to neutralize the action of plant laccase, phenoloxidase or peroxidase activities, which are increased in infected plants to evade fungal attack.
Autor/innen der BOKU Wien:
Felice Alfons Konrad
Klausberger Miriam
Ludwig Roland
Sygmund Christoph
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
DNA, Fungal/genetics;Fungal Proteins/genetics;Fungal Proteins/metabolism*;Glucose 1-Dehydrogenase/genetics;Glucose 1-Dehydrogenase/metabolism*;Phenols/metabolism*;Phyllachorales/enzymology*;Phyllachorales/genetics;Phylogeny;Plants/microbiology;Quinones/metabolism*;Sequence Analysis, DNA;Sequence Analysis, Protein;Substrate Specificity;



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