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Gewählte Publikation:

Mikula, H; Hametner, C; Berthiller, F; Warth, B; Krska, R; Adam, G; Frohlich, J.
(2012): Fast and reproducible chemical synthesis of zearalenone-14-beta,D-glucuronide
WORLD MYCOTOXIN J. 2012; 5(3): 289-296. FullText FullText_BOKU

Abstract:
The Fusarium mycotoxin zearalenone (ZEA) is mainly converted to the conjugate zearalenone-14-beta,D-glucuronide (ZEA-14-GlcA) during phase II detoxification in humans and animals. This metabolite - previously described as zearalenone-4-O-beta, D-glucuronide - is excreted via urine and could therefore serve as possible biomarker for ZEA exposure to estimate its intake. Direct determination of this substance is limited by the availability of a reference substance. So far, only the production of small amounts by enzymatic synthesis has been described. In this work, a fast and reproducible protocol for the chemical synthesis of ZEA-14-GlcA was developed, using substituted beta-resorcylic acid esters as mycotoxin mimics and different glucuronyl donors for optimising the glycosylation (Konigs-Knorr, trifluoroacetimidate method) and the deprotection step. This cost-effective procedure should be easily reproducible in other labs using standard equipment and common reagents.
Autor*innen der BOKU Wien:
Adam Gerhard
Berthiller Franz
Krska Rudolf
Warth Benedikt
BOKU Gendermonitor:


Find related publications in this database (Keywords)
mycotoxin conjugate
biomarker
synthesis
phase II metabolite
glucuronidation
ZEA-14-GlcA


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