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Gewählte Publikation:

Bueschl, C; Krska, R; Kluger, B; Schuhmacher, R; .
(2013): Isotopic labeling-assisted metabolomics using LC-MS.
Anal Bioanal Chem. 2013; 405(1):27-33 FullText FullText_BOKU

Metabolomics has emerged as the latest of the so-called "omics" disciplines and has great potential to provide deeper understanding of fundamental biochemical processes at the biological system level. Among recent technological developments, LC-HRMS enables determination of hundreds to thousands of metabolites over a wide range of concentrations and has developed into one of the most powerful techniques in non-targeted metabolomics. The analysis of mixtures of in-vivo-stable isotopic-labeled samples or reference substances with un-labeled samples leads to specific LC-MS data patterns which can be systematically exploited in practically all data-processing steps. This includes recognition of true metabolite-derived analytical features in highly complex LC-MS data and characterization of the global biochemical composition of biological samples. In addition, stable-isotopic labeling can be used for more accurate quantification (via internal standardization) and identification of compounds in different organisms.
Autor*innen der BOKU Wien:
Büschl Christoph
Kluger Bernhard
Krska Rudolf
Schuhmacher Rainer
Find related publications in this database (using NML MeSH Indexing)
Carbon Isotopes/chemistry
Chemistry Techniques, Analytical
Chromatography, Liquid/methods*
Isotope Labeling
Mass Spectrometry/methods*
Models, Statistical
Reproducibility of Results

Find related publications in this database (Keywords)
Bioanalytical methods
Mass spectrometry
Liquid chromatography

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