BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Bueschl, C; Krska, R; Kluger, B; Schuhmacher, R; .
(2013):
Isotopic labeling-assisted metabolomics using LC-MS.
Anal Bioanal Chem. 2013; 405(1):27-33
FullText
FullText_BOKU
- Abstract:
- Metabolomics has emerged as the latest of the so-called "omics" disciplines and has great potential to provide deeper understanding of fundamental biochemical processes at the biological system level. Among recent technological developments, LC-HRMS enables determination of hundreds to thousands of metabolites over a wide range of concentrations and has developed into one of the most powerful techniques in non-targeted metabolomics. The analysis of mixtures of in-vivo-stable isotopic-labeled samples or reference substances with un-labeled samples leads to specific LC-MS data patterns which can be systematically exploited in practically all data-processing steps. This includes recognition of true metabolite-derived analytical features in highly complex LC-MS data and characterization of the global biochemical composition of biological samples. In addition, stable-isotopic labeling can be used for more accurate quantification (via internal standardization) and identification of compounds in different organisms.
- Autor*innen der BOKU Wien:
- Büschl Christoph
- Kluger Bernhard
- Krska Rudolf
- Schuhmacher Rainer
- Find related publications in this database (using NML MeSH Indexing)
-
Carbon Isotopes/chemistry
Chemistry Techniques, Analytical
Chromatography, Liquid/methods*
Fusarium/metabolism
Humans
Isotope Labeling
Isotopes/chemistry*
Mass Spectrometry/methods*
Metabolomics/methods*
Models, Statistical
Reproducibility of Results
Software
- Find related publications in this database (Keywords)
- Bioanalytical methods
- Mass spectrometry
- Metabolomics
- Liquid chromatography
Altmetric: