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Gewählte Publikation:

Schweiger, W; Pasquet, JC; Nussbaumer, T; Paris, MP; Wiesenberger, G; Macadré, C; Ametz, C; Berthiller, F; Lemmens, M; Saindrenan, P; Mewes, HW; Mayer, KF; Dufresne, M; Adam, G; .
(2013): Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes.
Mol Plant Microbe Interact. 2013; 26(7):781-792 FullText FullText_BOKU

Abstract:
Plant small-molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant-pathogenic microorganisms. The ability to inactivate the Fusarium graminearum mycotoxin deoxynivalenol (DON) into DON-3-O-glucoside is crucial for resistance of cereals. We analyzed the UGT gene family of the monocot model species Brachypodium distachyon and functionally characterized two gene clusters containing putative orthologs of previously identified DON-detoxification genes from Arabidopsis thaliana and barley. Analysis of transcription showed that UGT encoded in both clusters are highly inducible by DON and expressed at much higher levels upon infection with a wild-type DON-producing F. graminearum strain compared with infection with a mutant deficient in DON production. Expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae revealed that only two B. distachyon UGT encoded by members of a cluster of six genes homologous to the DON-inactivating barley HvUGT13248 were able to convert DON into DON-3-O-glucoside. Also, a single copy gene from Sorghum bicolor orthologous to this cluster and one of three putative orthologs of rice exhibit this ability. Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity.
Autor*innen der BOKU Wien:
Adam Gerhard
Ametz Christian
Berthiller Franz
Lemmens Marc
Schweiger Wolfgang
Wiesenberger Gerlinde
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Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence;Brachypodium/enzymology*;Brachypodium/genetics;Fusarium/chemistry;Fusarium/pathogenicity*;Gene Dosage;Gene Expression Regulation, Plant;Gene Order;Glucosides/metabolism;Glycosyltransferases/genetics;Glycosyltransferases/metabolism*;Molecular Sequence Data;Multigene Family;Mutation;Mycotoxins/genetics;Mycotoxins/metabolism;Oryza sativa/enzymology;Oryza sativa/genetics;Phylogeny;Plant Diseases/microbiology*;Plant Proteins/genetics;Plant Proteins/metabolism;Saccharomyces cerevisiae/genetics;Saccharomyces cerevisiae/metabolism;Sorghum/enzymology;Sorghum/genetics;Species Specificity;Synteny;Trichothecenes/metabolism*;



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