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Gewählte Publikation:

Arcalis, E; Stadlmann, J; Rademacher, T; Marcel, S; Sack, M; Altmann, F; Stoger, E; .
(2013): Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins.
Plant Mol Biol. 2013; 83(1-2):105-117 FullText FullText_BOKU

Abstract:
Many plant-based systems have been developed as bioreactors to produce recombinant proteins. The choice of system for large-scale production depends on its intrinsic expression efficiency and its propensity for scale-up, post-harvest storage and downstream processing. Factors that must be considered include the anticipated production scale, the value and intended use of the product, the geographical production area, the proximity of processing facilities, intellectual property, safety and economics. It is also necessary to consider whether different species and organs affect the subcellular trafficking, structure and qualitative properties of recombinant proteins. In this article we discuss the subcellular localization and N-glycosylation of two commercially-relevant recombinant glycoproteins (Aspergillus niger phytase and anti-HIV antibody 2G12) produced in different plant species and organs. We augment existing data with novel results based on the expression of the same recombinant proteins in Arabidopsis and tobacco seeds, focusing on similarities and subtle differences in N-glycosylation that often reflect the subcellular trafficking route and final destination, as well as differences generated by unique enzyme activities in different species and tissues. We discuss the potential consequences of such modifications on the stability and activity of the recombinant glycoproteins.
Autor*innen der BOKU Wien:
Altmann Friedrich
Arcalis Elsa
Stadlmann Johannes
Stöger Eva
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
6-Phytase/genetics
6-Phytase/metabolism*
Antibodies, Monoclonal/genetics
Antibodies, Monoclonal/metabolism*
Arabidopsis/genetics
Arabidopsis/metabolism
Aspergillus niger/genetics
Aspergillus niger/metabolism
Broadly Neutralizing Antibodies
Fungal Proteins/genetics
Fungal Proteins/metabolism
Glycoproteins/genetics
Glycoproteins/metabolism*
Glycosylation
HIV Antibodies/genetics
HIV Antibodies/metabolism
Plant Leaves/genetics
Plant Leaves/metabolism*
Plants, Genetically Modified/genetics
Plants, Genetically Modified/metabolism
Polysaccharides/genetics
Polysaccharides/metabolism*
Protein Stability
Protein Transport
Recombinant Proteins/genetics
Recombinant Proteins/metabolism*
Seeds/genetics
Seeds/metabolism
Species Specificity
Tobacco/genetics
Tobacco/metabolism

Find related publications in this database (Keywords)
Molecular farming
Glycoprotein production
Plant-made pharmaceuticals
Protein trafficking
Subcellular deposition


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