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Prasetyo, EN; Rodriguez, RD; Lukesch, B; Weiss, S; Murkovic, M; Katsoyannos, E; Sygmund, C; Ludwig, R; Nyanhongo, GS; Guebitz, G.
(2015): Laccase-cellobiose dehydrogenase-catalyzed detoxification of phenolic-rich olive processing residues
INT J ENVIRON SCI TE. 2015; 12(4): 1343-1352. FullText FullText_BOKU

The combination of a laccase-hydroxybenzotriazole (HBT) mediator system with/without cellobiose dehydrogenase (CDH) or an additional Fenton reaction step for the elimination and/or detoxification of phenolic compounds in dry olive mill residues (DOR) and liquid olive mill wastewaters (OMW) was evaluated. The laccase-HBT-CDH and laccase-HBT-CDH-Fenton system were the most effective, removing at least 69 and 72 % of phenolic compounds from a total of 698 and 683 mg in OMW and DOR, respectively, in 12 h. The efficient removal of phenolic compounds was also accompanied by > 80 % reduction in biochemical oxygen demand and chemical oxygen demand in both DOR and OMW. Microbial community analysis using single-strand conformation polymorphism (SSCP) gels showed that biogas reactors supplemented with untreated and laccase-HBT-CDH-Fenton-treated DOR and OMW strongly inhibited growth of microorganisms. In contrast, the laccase-HBT- and laccase-HBT-CDH-pretreated OMW and DOR were detoxified as evidenced by SSCP analysis, which also indicated a distinct sensitivity of the individual members of the anaerobic population toward the toxicants. Further, although the laccase-HBT-CDH-Fenton system was effective in bleaching and removing phenolic compounds in both OMW and DOR, it was not able to support methane production. However, laccase-HBT and laccase-HBT-CDH indeed supported biogas production. This study therefore shows that the laccase-HBT-CDH system has a potential for the detoxification of olive mill residues, which can be potentially used as substrates for downstream processes.
Autor/innen der BOKU Wien:
Gübitz Georg
Ludwig Roland
Nyanhongo Gibson Stephen
Sygmund Christoph
BOKU Gendermonitor:

Find related publications in this database (Keywords)
Olive oil phenolic-rich waste
Cellobiose dehydrogenase
Fentonxxxs reaction
Single-strand conformation polymorphism
Microbial community analysis

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