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Gewählte Publikation:

Schneider, J; Castilho, A; Pabst, M; Altmann, F; Gruber, C; Strasser, R; Gattinger, P; Seifert, GJ; Steinkellner, H; .
(2015): Characterization of plants expressing the human β1,4-galactosyltrasferase gene.
Plant Physiol Biochem. 2015; 92:39-47 FullText FullText_BOKU

Abstract:
Modification of the plant N-glycosylation pathway towards human type structures is an important strategy to implement plants as expression systems for therapeutic proteins. Nevertheless, relatively little is known about the overall impact of non-plant glycosylation enzymes in stable transformed plants. Here, we analyzed transgenic lines (Nicotiana benthamiana and Arabidopsis thaliana) that stably express a modified version of human beta 1,4-galactosyltransferase ((ST)GalT). While some transgenic plants grew normally, other lines exhibited a severe phenotype associated with stunted growth and developmental retardation. The severity of the phenotype correlated with both increased (ST)GalT mRNA and protein levels but no differences Were observed between N-glycosylation profiles of plants with and without the phenotype. In contrast to non-transgenic plants, all (ST)GalT expressing plants synthesized significant amounts of incompletely processed (largely depleted of core fucose) N-glycans with up to 40% terminally galactosylated structures. While transgenic plants showed no differences in nucleotide sugar composition and cell wall monosaccharide content, alterations in the reactivity of cell wall carbohydrate epitopes associated with arabinogalactan-proteins and pectic homogalacturonan were detected in (ST)GalT expressing plants. Notably, plants with phenotypic alterations showed increased levels of hydrogen peroxide, most probably a consequence of hypersensitive reactions. Our data demonstrate that unfavorable phenotypical modifications may occur upon stable in planta expression of non-native glycosyltransferases. Such important issues need to be taken into consideration in respect to stable glycan engineering in plants. (C) 2015 The Authors. Published by Elsevier Masson SAS.
Autor/innen der BOKU Wien:
Altmann Friedrich
De Vitoria Pereira Alexandra
Gattinger Pia Simone
Grünwald-Gruber Clemens
Pabst Martin
Seifert Georg
Steinkellner Herta
Strasser Richard
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Find related publications in this database (Keywords)
beta 1,4-Galactosylation
Glyco-engineering
Nicotiana benthamiana
Transgenic plants
Developmental phenotype


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