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Gewählte Publikation:

Zweckmair, T; Schiehser, S; Rosenau, T; Potthast, A.
(2017): Improved quantification of monosaccharides in complex lignocellulosic biomass matrices: A gas chromatography-mass spectrometry based approach
CARBOHYD RES. 2017; 446: 7-12. FullText FullText_BOKU

Abstract:
A novel method for the precise and accurate quantification of wood monosaccharides by gas chromatography mass-spectrometry in complex lignocellulosic biomass matrices is presented. Instead of using the syn- or the anti-peak obtained by blocking the anomeric center using e.g. oximation, the sum of each syn- and anti-peak pair is used for quantification rendering the approach of the apparently constant syn- and anti-peak area ratio. Each wood monosaccharide syn- and anti-peak could essentially be distinguished upon 0-ethoximation followed by trifluoroacetylation and separation on a 14% cyanopropyl/phenyl- 86% dimethylpoly-siloxane column. Additionally, internal standardization was carried out applying isotopically labeled compounds. Hence, the analytical figures of merit, such as precision and accuracy, could be substantially improved compared to standard gas-chromatography mass spectrometry as well as high-performance anion exchange chromatography (HPAEC) coupled to pulsedamperometric detection (PAD). The applicability of the novel gas-chromatography mass-spectrometry approach is demonstrated by analysis of five selected lignocellulosic biomass sample matrices typically occurring in a biorefinery scenario. Even in exceedingly complex sample matrices precise and accurate data are obtained according to the methodology presented. (C) 2017 Elsevier Ltd. All rights reserved.
Autor/innen der BOKU Wien:
Potthast Antje
Rosenau Thomas
Schiehser Sonja
Zweckmair Thomas
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Gas-chromatography mass-spectrometry
Monosaccharides
O-ethoximation trifluoroacetylation
Isotopically labeled internal standards
Lignocellulosic biomass


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