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Gewählte Publikation:

Lorenzo, K; Ton, P; Clark, JL; Coulibaly, S; Mach, L.
(2000): Invasive properties of murine squamous carcinoma cells: secretion of matrix-degrading cathepsins is attributable to a deficiency in the mannose 6-phosphate/insulin-like growth factor II receptor.
Cancer Res. 2000; 60(15):4070-4076

Penetration of basement membrane layers is a hallmark feature of metastatic tumor cells. The invasive propensity of murine SCC-VII squamous carcinoma cells is in part attributable to the extracellular action of the lysosomal cysteine proteinase cathepsin B. Although most noncancerous cells store this enzyme in the lysosomes, we found that SCC-VII cells release a large fraction (42%) of their newly synthesized procathepsin B into the culture medium. Procathepsins D and L, the precursors of other major lysosomal proteinases, are also secreted in significant amounts (24 and 75%, respectively). In contrast, normal murine 3T3-L1 fibroblasts exocytose only minor amounts of their newly synthesized procathepsins B (10%), D (<1%), and L (16%). Western blotting analysis revealed that SCC-VII cells are deficient in the 300 kDa mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGF2R), a tumor suppressor with a central role in the intracellular transport of lysosomal enzymes. Consistent with the absence of M6P/IGF2R, SCC-VII cells lack dense lysosomes, with the bulk of intracellular acid hydrolases residing in late endosomes/prelysosomes. On the other hand, the synthesis of the M6P recognition marker on lysosomal enzymes is not impaired in SCC-VII cells, because [P-33]P-i is incorporated into the carbohydrate moieties of procathepsins B, D, and L. Furthermore, 69% of the phosphorylated N-linked oligosaccharides synthesized by SCC-VII cells carry phosphomonoester groups and as such constitute high-affinity ligands for M6P receptors. SCC-VII cells express the 46 kDa cation-dependent M6P receptor (MPR46), but intracellular retention of procathepsins B, D, and L is not affected by ammonium chloride and chloroquine, agents known to perturb the M6P receptor system. Our results indicate that failure to express the 300 kDa M6P/IGF2R may enhance the metastatic capacity of tumor cells by inducing the secretion of procathepsins B, D, and L.
Autor*innen der BOKU Wien:
Mach Lukas
Find related publications in this database (using NML MeSH Indexing)
3T3 Cells - metabolism
Animals - metabolism
Carcinoma, Squamous Cell - metabolism
Cathepsin B - biosynthesis
Cathepsin D - biosynthesis
Cathepsins - biosynthesis
Cysteine Endopeptidases - biosynthesis
Endopeptidases - biosynthesis
Humans - biosynthesis
Lysosomes - enzymology
Mice - enzymology
Neoplasm Invasiveness - enzymology
Phosphorylation - enzymology
Receptor, IGF Type 2 - biosynthesis
Tumor Cells, Cultured - biosynthesis

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