BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Dirnberger, D; Bencúr, P; Mach, L; Steinkellner, H.
(2002): The Golgi localization of Arabidopsis thaliana beta1,2-xylosyltransferase in plant cells is dependent on its cytoplasmic and transmembrane sequences.
Plant Mol Biol. 2002; 50(2):273-281

To investigate the targeting of proteins to the plant Golgi we studied Arabidopsis thaliana beta1,2-xylosyltransferase (XylT), a glycosyltransferase which is unique to plants and some invertebrates. Different deletion constructs of the putative cytoplasmic (C)-transmembrane (T)-stem (S) region of the enzyme were transiently expressed in the tobacco-related model plant species Nicotiana benthamiana. Subcellular localization of fusion proteins between CTS, CT, T, or C domains and the reporter molecule green fluorescent protein by fluorescence microcopy and density-gradient centrifugation revealed that the CT region alone is sufficient to sustain Golgi retention of XylT without the contribution of any luminal sequences. The finding of an incomplete retention by the T region alone suggests an important auxiliary role of the C domain in Golgi retention of the protein. However, the C segment did not confer any Golgi retention by itself, as the respective fusion protein was found exclusively in the cytoplasm. These results provide evidence that plant and mammalian cells rely on similar mechanisms to deliver glycosyltransferases to the Golgi apparatus.
Autor*innen der BOKU Wien:
Mach Lukas
Steinkellner Herta
Find related publications in this database (using NML MeSH Indexing)
Arabidopsis - enzymology
Arabidopsis Proteins - genetics
Blotting, Western - genetics
Centrifugation, Density Gradient - genetics
Gene Expression Regulation, Plant - genetics
Golgi Apparatus - enzymology
Green Fluorescent Proteins - enzymology
Luminescent Proteins - genetics
Membrane Glycoproteins - genetics
Microscopy, Fluorescence - genetics
Pentosyltransferases - genetics
Plants, Genetically Modified - genetics
Recombinant Fusion Proteins - genetics
Tobacco - cytology

Find related publications in this database (Keywords)
Golgi apparatus
N-glycan biosynthesis
beta 1,2-xylosyltransferase

© BOKU Wien Impressum