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Gewählte Publikation:

Schähs, M; Strasser, R; Stadlmann, J; Kunert, R; Rademacher, T; Steinkellner, H.
(2007): Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern.
Plant Biotechnol J. 2007; 5(5):657-663 FullText FullText_BOKU

in recent years, plants have become an attractive alternative for the production of recombinant proteins. However, their inability to perform authentic mammalian N-glycosylation may cause limitations for the production of therapeutics. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex Winked glycans, as these N-glycan epitopes are immunogenic in mammals. In our attempts towards the humanization of plant N-glycans, we have generated an Arabidopsis thaliana knockout line that synthesizes complex N-glycans lacking immunogenic xylose and fucose epitopes. Here, we report the expression of a monoclonal antibody in these glycan-engineered plants that carry a homogeneous mammalian-like complex N-glycan pattern without beta 1,2-xylose and core alpha 1,3-fucose. Plant and Chinese hamster ovary (CHO)-derived immunoglobulins (IgGs) exhibited no differences in electrophoretic mobility and enzyme-linked immunosorbent specificity assays. Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform.
Autor*innen der BOKU Wien:
Kunert Renate
Stadlmann Johannes
Steinkellner Herta
Strasser Richard
Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies, Monoclonal - genetics
Arabidopsis - genetics
CHO Cells - genetics
Cricetinae - genetics
Cricetulus - genetics
Enzyme-Linked Immunosorbent Assay - genetics
Epitopes - chemistry
Gene Deletion - chemistry
Glycosylation - chemistry
Humans - chemistry
Immunoglobulin G - immunology
Plant Leaves - genetics
Plants, Genetically Modified - genetics
Polysaccharides - chemistry
Recombinant Proteins - immunology

Find related publications in this database (Keywords)
beta 1,2-xylose and core alpha 1,3-fucose
plant glycan
recombinant proteins

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