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Gewählte Publikation:

Strasser, R; Bondili, JS; Vavra, U; Schoberer, J; Svoboda, B; Glössl, J; Léonard, R; Stadlmann, J; Altmann, F; Steinkellner, H; Mach, L; .
(2007): A unique beta1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana.
Plant Cell. 2007; 19(7):2278-2292 FullText FullText_BOKU

Abstract:
In plants, the only known outer-chain elongation of complex N-glycans is the formation of Lewis a [Fuc alpha 1-4(Galb1-3) GlcNAc-R] structures. This process involves the sequential attachment of beta 1,3-galactose and alpha 1,4-fucose residues by b1,3galactosyltransferase and alpha 1,4-fucosyltransferase. However, the exact mechanism underlying the formation of Lewis a epitopes in plants is poorly understood, largely because one of the involved enzymes, beta 1,3-galactosyltransferase, has not yet been identified and characterized. Here, we report the identification of an Arabidopsis thaliana beta 1,3-galactosyltransferase involved in the biosynthesis of the Lewis a epitope using an expression cloning strategy. Overexpression of various candidates led to the identification of a single gene (named GALACTOSYLTRANSFERASE1 [GALT1]) that increased the originally very low Lewis a epitope levels in planta. Recombinant GALT1 protein produced in insect cells was capable of transferring beta 1,3-linked galactose residues to various N-glycan acceptor substrates, and subsequent treatment of the reaction products with alpha 1,4-fucosyltransferase resulted in the generation of Lewis a structures. Furthermore, transgenic Arabidopsis plants lacking a functional GALT1 mRNA did not show any detectable amounts of Lewis a epitopes on endogenous glycoproteins. Taken together, our results demonstrate that GALT1 is both sufficient and essential for the addition of beta 1,3-linked galactose residues to N-glycans and thus is required for the biosynthesis of Lewis a structures in Arabidopsis. Moreover, cell biological characterization of a transiently expressed GALT1-fluorescent protein fusion using confocal laser scanning microscopy revealed the exclusive location of GALT1 within the Golgi apparatus, which is in good agreement with the proposed physiological action of the enzyme.
Autor*innen der BOKU Wien:
Altmann Friedrich
Glößl Josef
Leonard Renaud
Mach Lukas
Schoberer Jennifer
Stadlmann Johannes
Steinkellner Herta
Strasser Richard
Vavra Ulrike
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence;Animals;Arabidopsis/enzymology*;Arabidopsis/genetics;Arabidopsis Proteins/chemistry;Arabidopsis Proteins/genetics;Arabidopsis Proteins/metabolism*;Carbohydrate Conformation*;DNA, Bacterial;Epitopes/chemistry;Galactosyltransferases/chemistry;Galactosyltransferases/genetics;Galactosyltransferases/metabolism*;Gene Expression Regulation, Enzymologic;Gene Expression Regulation, Plant;Genes, Plant;Glycosylation;Insects;Molecular Sequence Data;Mutagenesis, Insertional;Oligosaccharides/chemistry*;Phylogeny;Plant Leaves/enzymology;Polysaccharides/biosynthesis*;Polysaccharides/chemistry*;Protein Transport;RNA Interference;Recombinant Proteins/metabolism;Subcellular Fractions/enzymology;Substrate Specificity;



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