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Gewählte Publikation:

Sefc, KM; Leonhardt, W; Steinkellner, H.
(2000): Partial sequence identification of grapevine-leafroll-associated virus-1 and development of a highly sensitive IC-RT-PCR detection method
J VIROL METH. 2000; 86(1): 101-106.

Using immunocapture reverse transcription PCR (IC-RT-PCR) a specific PCR product from GLRaV-1 infected vine samples was amplified with the help of degenerate primers deduced from the conserved HSP70 region of closteroviruses. 511 basepairs of the 5' end of GLRaV-1 HSP70 gene were identified. Within this region, putative GLRaV-1 specific primers were designed and an IC RT-PCR detection procedure was developed which is about 125 times more sensitive than the established ELISA method. No PCR product was amplified in GLRaV-2,-3 and -4 infected plants which indicates the specificity of the primers. This procedure may serve as an alternative method for GLRaV-1 detection where the sensitivity of ELISA is insufficient. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
Autor*innen der BOKU Wien:
Steinkellner Herta

Find related publications in this database (Keywords)
immunocapture reverse transcription PCR
grapevine-leafroll-associated virus-1

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