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Gewählte Publikation:

Huang, F; Zago, MK; Abas, L; van Marion, A; Galvn-Ampudia, CS; Offringa, R.
(2010): Phosphorylation of conserved PIN motifs directs Arabidopsis PIN1 polarity and auxin transport.
Plant Cell. 2010; 22(4):1129-1142 FullText FullText_BOKU

Polar cell-to-cell transport of auxin by plasma membrane-localized PIN-FORMED (PIN) auxin efflux carriers generates auxin gradients that provide positional information for various plant developmental processes. The apical-basal polar localization of the PIN proteins that determines the direction of auxin flow is controlled by reversible phosphorylation of the PIN hydrophilic loop (PINHL). Here, we identified three evolutionarily conserved TPRXS(N/S) motifs within the PIN1HL and proved that the central Ser residues were phosphorylated by the PINOID (PID) kinase. Loss-of-phosphorylation PIN1: green fluorescent protein (GFP) (Ser to Ala) induced inflorescence defects, correlating with their basal localization in the shoot apex, and induced internalization of PIN1: GFP during embryogenesis, leading to strong embryo defects. Conversely, phosphomimic PIN1: GFP (Ser to Glu) showed apical localization in the shoot apex but did not rescue pin1 inflorescence defects. Both loss-of-phosphorylation and phosphomimic PIN1: GFP proteins were insensitive to PID overexpression. The basal localization of loss-of-phosphorylation PIN1: GFP increased auxin accumulation in the root tips, partially rescuing PID overexpression-induced root collapse. Collectively, our data indicate that reversible phosphorylation of the conserved Ser residues in the PIN1HL by PID (and possibly by other AGC kinases) is required and sufficient for proper PIN1 localization and is thus essential for generating the differential auxin distribution that directs plant development.
Autor*innen der BOKU Wien:
Abas Melinda Frances Anna
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Arabidopsis - genetics
Arabidopsis Proteins - genetics
DNA, Plant - genetics
Gene Expression Regulation, Developmental -
Gene Expression Regulation, Plant -
Indoleacetic Acids - metabolism
Inflorescence - growth & development
Membrane Transport Proteins - genetics
Molecular Sequence Data -
Phosphorylation -
Plant Growth Regulators - metabolism
Protein Interaction Domains and Motifs -
Protein-Serine-Threonine Kinases - genetics
Sequence Alignment -

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