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Gewählte Publikation:

Schoberer, J; Runions, J; Steinkellner, H; Strasser, R; Hawes, C; Osterrieder, A.
(2010): Sequential Depletion and Acquisition of Proteins during Golgi Stack Disassembly and Reformation
TRAFFIC. 2010; 11(11): 1429-1444. FullText FullText_BOKU

Abstract:
Herein, we report the stepwise transport of multiple plant Golgi membrane markers during disassembly of the Golgi apparatus in tobacco leaf epidermal cells in response to the induced expression of the GTP-locked Sar1p or Brefeldin A (BFA), and reassembly on BFA washout. The distribution of fluorescent Golgi-resident N-glycan processing enzymes and matrix proteins (golgins) with specific cis-trans-Golgi sub-locations was followed by confocal microscopy during disassembly and reassembly. The first event during Golgi disassembly was the loss of trans-Golgi enzymes and golgins from Golgi membranes, followed by a sequential redistribution of medial and cis-Golgi enzymes into the endoplasmic reticulum (ER), whilst golgins were relocated to the ER or cytoplasm. This event was confirmed by fractionation and immuno-blotting. The sequential redistribution of Golgi components in a trans-cis sequence may highlight a novel retrograde trafficking pathway between the trans-Golgi and the ER in plants. Release of Golgi markers from the ER upon BFA washout occurred in the opposite sequence, with cis-matrix proteins labelling Golgi-like structures before cis/medial enzymes. Trans-enzyme location was preceded by trans-matrix proteins being recruited back to Golgi membranes. Our results show that Golgi disassembly and reassembly occur in a highly ordered fashion in plants.
Autor*innen der BOKU Wien:
Schoberer Jennifer
Steinkellner Herta
Strasser Richard

Find related publications in this database (Keywords)
Brefeldin A
golgins
Golgi apparatus
N-glycan processing enzymes
Sar1p GTPase


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