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Gewählte Publikation:

Marqus-Bueno, MM; Moreno-Romero, J; Abas, L; De Michele, R; Martnez, MC.
(2011): A dominant negative mutant of protein kinase CK2 exhibits altered auxin responses in Arabidopsis.
Plant J. 2011; 67(1):169-180 FullText FullText_BOKU

Protein kinase CK2 is a pleiotropic Ser/Thr kinase, evolutionary conserved in eukaryotes. Studies performed in different organisms, from yeast to humans, have highlighted the importance of CK2 in cell growth and cell-cycle control. However, the signalling pathways in which CK2 is involved have not been fully identified. In plants, the phytohormone auxin is a major regulator of cell growth. Recent discoveries have demonstrated that differential distribution of within auxin plant tissues is essential for developmental processes, and that this distribution is dependent on polar auxin transport. We report here that a dominant-negative mutant of CK2 (CK2mut) in Arabidopsis thaliana shows phenotypic traits that are typically linked to alterations in auxin-dependent processes. However, CK2mut plants exhibit normal responses to exogenous indole-3-acetic acid (IAA) indicating that they are not affected in the perception of the hormone but upstream in the pathway. We demonstrate that mutant plants are not deficient in IAA but are impaired in its transport. Using genetic and pharmacological tools we show that CK2 activity depletion hinders correct formation of auxin gradients and leads to widespread changes in the expression of auxin-related genes. In particular, members of the auxin efflux carrier family (PINs), and the protein kinase PINOID, both key regulators of auxin fluxes, were misexpressed. PIN4 and PIN7 were also found mislocalized, with accumulation in endosomal bodies. We propose that CK2 functions in the regulation of auxin-signalling pathways, particularly in auxin transport.
Autor*innen der BOKU Wien:
Abas Melinda Frances Anna
Find related publications in this database (using NML MeSH Indexing)
Arabidopsis - enzymology
Arabidopsis Proteins - genetics
Biological Transport -
Casein Kinase II - genetics
DNA, Plant - genetics
Gene Expression Regulation, Developmental -
Gene Expression Regulation, Enzymologic -
Gene Expression Regulation, Plant -
Genes, Reporter -
Indoleacetic Acids - metabolism
Membrane Transport Proteins - metabolism
Mutation -
Phenotype -
Plant Growth Regulators - metabolism
Plant Roots - enzymology
Plants, Genetically Modified - enzymology
Protein-Serine-Threonine Kinases - metabolism
RNA, Plant - genetics
Seedling - enzymology
Signal Transduction -

Find related publications in this database (Keywords)
protein kinase CK2
root development
lateral roots
cell division cycle

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