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Gewählte Publikation:

Farid, A; Pabst, M; Schoberer, J; Altmann, F; Glössl, J; Strasser, R; .
(2011): Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth.
Plant J. 2011; 68(2):314-325 FullText FullText_BOKU

Abstract:
Assembly of the dolichol-linked oligosaccharide precursor (Glc(3)Man(9)GlcNAc(2)) is highly conserved among eukaryotes. In contrast to yeast and mammals, little is known about the biosynthesis of dolichol-linked oligosaccharides and the transfer to asparagine residues of nascent polypeptides in plants. To understand the biological function of these processes in plants we characterized the Arabidopsis thaliana homolog of yeast ALG10, the alpha 1,2-glucosyltransferase that transfers the terminal glucose residue to the lipid-linked precursor. Expression of an Arabidopsis ALG10-GFP fusion protein in Nicotiana benthamiana leaf epidermal cells revealed a reticular distribution pattern resembling endoplasmic reticulum (ER) localization. Analysis of lipid-linked oligosaccharides showed that Arabidopsis ALG10 can complement the yeast Delta lg10 mutant strain. A homozygous Arabidopsis T-DNA insertion mutant (alg10-1) accumulated mainly lipid-linked Glc(2)Man(9)Glc-NAc(2) and displayed a severe protein underglycosylation defect. Phenotypic analysis of alg10-1 showed that mutant plants have altered leaf size when grown in soil. Moreover, the inactivation of ALG10 in Arabidopsis resulted in the activation of the unfolded protein response, increased salt sensitivity and suppression of the phenotype of a-glucosidase I-deficient plants. In summary, these data show that Arabidopsis ALG10 is an ER-resident a1,2-glucosyltransferase that is required for lipid-linked oligosaccharide biosynthesis and subsequently for normal leaf development and abiotic stress response.
Autor*innen der BOKU Wien:
Altmann Friedrich
Glößl Josef
Pabst Martin
Schoberer Jennifer
Strasser Richard
Find related publications in this database (using NML MeSH Indexing)
Arabidopsis/embryology;Arabidopsis/enzymology*;Arabidopsis/genetics;Arabidopsis/growth & development;Arabidopsis Proteins/genetics;Arabidopsis Proteins/metabolism*;Carbohydrate Sequence;Endoplasmic Reticulum/enzymology;Endoplasmic Reticulum/metabolism;Genetic Complementation Test;Glucosyltransferases/genetics;Glucosyltransferases/metabolism;Glycosylation;Green Fluorescent Proteins;Molecular Sequence Data;Mutagenesis, Insertional;Oligosaccharides/biosynthesis*;Oligosaccharides/chemistry;Phenotype;Plant Leaves/enzymology;Plant Leaves/genetics;Plant Leaves/growth & development*;Plant Leaves/metabolism;Polyisoprenyl Phosphate Sugars/biosynthesis*;Polyisoprenyl Phosphate Sugars/chemistry;Polysaccharides/metabolism;Protein Processing, Post-Translational;Saccharomyces cerevisiae/genetics;Saccharomyces cerevisiae/metabolism;Salt-Tolerance;Stress, Physiological;Tobacco/genetics;Tobacco/metabolism;Unfolded Protein Response;

Find related publications in this database (Keywords)
protein glycosylation
glycosyltransferase
lipid-linked oligosaccharides
posttranslational modification
endoplasmic reticulum
abiotic stress


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