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Gewählte Publikation:

Staudacher, E; Altmann, F; Glössl, J; März, L; Schachter, H; Kamerling, JP; Hård, K; Vliegenthart, JF.
(1991): GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1----6GlcNAc)-Asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.
Eur J Biochem. 1991; 199(3):745-751 FullText FullText_BOKU

Incubation of honeybee (Apis mellifica) venom-gland extracts with GDP-[C-14]fucose and GlcNAc-beta-1 --> 2Man-alpha-1 --> 6(GlcNAc-beta-1 --> 2Man-alpha-1 --> 3)Man-beta-1 --> 4GlcNAc-beta-1 --> 4(Fuc-alpha-1 --> 6)GlcNAc-beta-1 --> N-Asn-peptide(NAc) gave a labeled product in 40% yield. Analysis by 500-MHz H-1-NMR spectroscopy indicated the transferred fucose (Fuc) residue to be alpha-1 --> 3-linked to the Asn-bound GlcNAc. Further proof was provided by one-dimensional and two-dimensional H-1-NMR analysis of the incubation mixture, after incubation with beta-N-acetylhexosaminidase. The established carbohydrate structure [GRAPHICS] proves the existence of a novel alpha-1 --> 3-fucosyltransferase with the ability to effect difucosylation of the Asn-bound GlcNAc in N-glycans.
Autor*innen der BOKU Wien:
Altmann Friedrich
Glößl Josef
März Leopold
Staudacher Erika
Find related publications in this database (using NML MeSH Indexing)
Acetylglucosamine - metabolism
Animals -
Bee Venoms -
Bees - enzymology
Carbohydrate Conformation -
Carbohydrate Sequence -
Fucosyltransferases - isolation & purification
Guanosine Diphosphate Fucose - metabolism
Kinetics -
Magnetic Resonance Spectroscopy - methods
Molecular Sequence Data -
Substrate Specificity -

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