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Gewählte Publikation:

Shigeta, K; Koellensperger, G; Rampler, E; Traub, H; Rottmann, L; Panne, U; Okino, A; Jakubowski, N.
(2013): Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field mass spectrometer for label-free detection of trace elements
J ANAL ATOM SPECTROM. 2013; 28(5): 637-645. FullText FullText_BOKU

We have applied a micro droplet generator (mu DG) for sample introduction of single selenized yeast cells into a sector field ICP-MS, which was operated in a fast scanning mode with sampling rates of up to 10 kHz, to measure single cells time resolved with 100 mu s integration time. Selenized yeast cells have been used as a model system for preliminary investigation. The single cells to be measured have been embedded into droplets and it will be shown that the time duration of a single cell event always is about 400 to 500 ms, and thus comparable to the time duration of a droplet without a cell. A fixed droplet generation rate of 50 Hz produced equidistant signals in time of each droplet event and was advantageous to separate contribution from background and blank from the analytical signal. Open vessel digestion and a multielement analysis were performed with washed yeast cells and absolute amounts per single cell were determined for Na (0.91 fg), Mg (9.4 fg), Fe (5.9 fg), Cu (0.54 fg), Zn (1.2 fg) and Se (72 fg). Signal intensities from single cells have been measured for the elements Cu, Zn and Se, and histograms were calculated for about 1000 cell events. The mean elemental sensitivities measured here range from 0.7 counts per ag (Se) to 10 counts per ag (Zn) with RSDxxxs from 49% (Zn) to 69% (Se) for about 1000 cell events.
Autor/innen der BOKU Wien:
Köllensperger Gunda
Rampler Evelyn
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