BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Kurz, S; Jin, C; Hykollari, A; Gregorich, D; Giomarelli, B; Vasta, GR; Wilson, IB; Paschinger, K; .
(2013):
Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans.
J Biol Chem. 2013; 288(34):24410-24428
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- Abstract:
- The eastern oyster (Crassostrea virginica) has become a useful model system for glycan-dependent host-parasite interactions due to the hijacking of the oyster galectin CvGal1 for host entry by the protozoan parasite Perkinsus marinus, the causative agent of Dermo disease. In this study, we examined the N-glycans of both the hemocytes, which via CvGal1 are the target of the parasite, and the plasma of the oyster. In combination with HPLC fractionation, exoglycosidase digestion, and fragmentation of the glycans, mass spectrometry revealed that the major N-glycans of plasma are simple hybrid structures, sometimes methylated and core alpha 1,6-fucosylated, with terminal alpha 1,3-linked galactose; a remarkable high degree of sulfation of such glycans was observed. Hemocytes express a larger range of glycans, including core-difucosylated paucimannosidic forms, whereas bi- and triantennary glycans were found in both sources, including structures carrying sulfated and methylated variants of the histo-blood group A epitope. The primary features of the oyster whole hemocyte N-glycome were also found in dominin, the major plasma glycoprotein, which had also been identified as a CvGal1 glycoprotein ligand associated with hemocytes. The occurrence of terminal blood group moieties on oyster dominin and on hemocyte surfaces can account in part for their affinity for the endogenous CvGal1.
- Autor*innen der BOKU Wien:
- Hykollari Alba
- Kurz Simone
- Paschinger Katharina
- Wilson Iain B.H.
- Find related publications in this database (using NML MeSH Indexing)
-
ABO Blood-Group System/chemistry
ABO Blood-Group System/metabolism*
Alveolata/physiology
Animals
Blood Proteins/chemistry
Blood Proteins/metabolism*
Crassostrea/chemistry
Crassostrea/metabolism*
Crassostrea/parasitology
Epitopes/chemistry
Epitopes/metabolism
Galectins/chemistry
Galectins/metabolism*
Hemocytes/chemistry
Hemocytes/metabolism*
Hemocytes/parasitology
Host-Parasite Interactions/physiology
Polysaccharides/chemistry
Polysaccharides/metabolism*
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