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Gewählte Publikation:

Castillo, NI; Ibanez, M; Beltran, E; Rivera-Monroy, J; Ochoa, JC; Paez-Castillo, M; Posada-Buitrago, ML; Sulyok, M; Hernandez, F.
(2016): Identification of mycotoxins by UHPLC-QTOF MS in airborne fungi and fungi isolated from industrial paper and antique documents from the Archive of Bogota
ENVIRON RES. 2016; 144: 130-138. FullText FullText_BOKU

Mold deterioration of historical documents in archives and libraries is a frequent and complex phenomenon that may have important economic and cultural consequences. In addition, exposure to toxic fungal metabolites might produce health problems. In this work, samples of broths of fungal species isolated from the documentary material and from indoor environmental samples of the Archive of Bogota have been analyzed to investigate the presence of mycotoxins. High resolution mass spectrometry made possible to search for a large number of mycotoxins, even without reference standards available at the laboratory. For this purpose, a screening strategy based on ultra-high pressure liquid chromatography coupled to quadrupole-time of flight mass spectrometry (UHPLC-QTOF MS) under MSE mode was applied. A customized home-made database containing elemental composition for around 600 mycotoxins was compiled. The presence of the (de)protonated molecule measured at its accurate mass was evaluated in the samples. When a peak was detected, collision induced dissociation fragments and characteristic isotopic ions were also evaluated and used for tentative identification, based on structure compatibility and comparison with literature data (if existing). Up to 44 mycotoxins were tentatively identified by UHPLC-QTOF MS. 34 of these tentative compounds were confirmed by subsequent analysis using a targeted LC-MS/MS method, supporting the strong potential of QTOF MS for identification/elucidation purposes. The presence of mycotoxins in these samples might help to reinforce safety measures for researchers and staff who work on reception, restoration and conservation of archival material, not only at the Archive of Bogota but worldwide. (C) 2015 Elsevier Inc. All rights reserved.
Autor*innen der BOKU Wien:
Sulyok Michael
Find related publications in this database (using NML MeSH Indexing)
Air Pollution, Indoor;Archives;Chromatography, High Pressure Liquid;Colombia;DNA, Fungal/analysis;DNA, Ribosomal/analysis;Environmental Monitoring;Environmental Pollutants/analysis*;Fungi/genetics;Fungi/isolation & purification;Mycotoxins/analysis*;Paper;Sequence Analysis, DNA;Tandem Mass Spectrometry;

Find related publications in this database (Keywords)
Liquid chromatography
Quadrupole time-of-flight mass spectrometry

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