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Gewählte Publikation:

Woegerbauer, M; Zeinzinger, J; Gottsberger, RA; Pascher, K; Hufnagl, P; Indra, A; Fuchs, R; Hofrichter, J; Kopacka, I; Korschineck, I; Schleicher, C; Schwarz, M; Steinwider, J; Springer, B; Allerberger, F; Nielsen, KM; Fuchs, K.
(2015): Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria
ENVIRON POLLUT. 2015; 206: 342-351. FullText FullText_BOKU

Abstract:
Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3xxx)-IIa/nptII and aph(3xxx)-IIIa/nptIII frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median: 150 copies/g soil; range: 31-856) and 85% for nptIII (1190 copies/g soil; 13-61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval: 0-33%) were positive for nptII, none for nptIII (0-0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems. (C) 2015 Elsevier Ltd. All rights reserved.
Autor/innen der BOKU Wien:
Pascher Kathrin
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Resistance
Aminoglycosides
Antibiotics
Soil contamination
Real time PCR


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