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Gewählte Publikation:

Elefant, GR; Roldan, WH; Seebock, A; Kosma, P.
(2016): Evaluation of a di-O-methylated glycan as a potential antigenic target for the serodiagnosis of human toxocariasis
PARASITE IMMUNOL. 2016; 38(4): 236-243. FullText FullText_BOKU

Abstract:
Serodiagnosis of human toxocariasis is based on the detection of specific IgG antibodies by the enzyme-linked immunosorbent assay (ELISA) using Toxocara larvae excretory-secretory (TES) antigens, but its production is a laborious and time-consuming process being also limited by the availability of adult females of T.canis as source for ova to obtain larvae. Chemical synthesis of the di-O-methylated (DiM) glycan structure found in the TES antigens has provided material for studying the antibody reactivity in a range of mammalian hosts, showing reactivity with human IgM and IgG. In this study, we have evaluated the performance of the DiM glycan against a panel of sera including patients with toxocariasis (n=60), patients with other helminth infections (n=75) and healthy individuals (n=94), showing that DiM is able to detect IgG antibodies with a sensitivity and specificity of 917% and 947%, respectively, with a very good agreement with the TES antigens (kappa = 0825). However, cross-reactivity was observed in some sera from patients with ascariasis, hymenolepiasis and fascioliasis. These results show that the DiM glycan could be a promising antigenic tool for the serodiagnosis of human toxocariasis.
Autor*innen der BOKU Wien:
Kosma Paul
BOKU Gendermonitor:


Find related publications in this database (Keywords)
enzyme-linked immunosorbent assay
excretory-secretory antigen
immunoglobulin
serodiagnosis
toxocara canis
toxocariasis


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