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Gewählte Publikation:

Masching, S; Naehrer, K; Schwartz-Zimmermann, HE; Sarandan, M; Schaumberger, S; Dohnal, I; Nagl, V; Schatzmayr, D.
(2016): Gastrointestinal Degradation of Fumonisin B-1 by Carboxylesterase FumD Prevents Fumonisin Induced Alteration of Sphingolipid Metabolism in Turkey and Swine
TOXINS. 2016; 8(3): FullText FullText_BOKU

Abstract:
The mycotoxin fumonisin B-1 (FB1) is a frequent contaminant of feed and causes various adverse health effects in domestic animals. Hence, effective strategies are needed to prevent the impact of fumonisins on livestock productivity. Here we evaluated the capability of the fumonisin carboxylesterase FumD to degrade FB1 to its less toxic metabolite hydrolyzed FB1 (HFB1) in the gastrointestinal tract of turkeys and pigs. First, an ex vivo pig model was used to examine the activity of FumD under digestive conditions. Within 2 h of incubation with FumD, FB1 was completely degraded to HFB1 in the duodenum and jejunum, respectively. To test the efficacy of the commercial application of FumD (FUMzyme) in vivo, female turkeys (n = 5) received either basal feed (CON), fumonisin-contaminated feed (15 mg/kg FB1+FB2; FB) or fumonisin-contaminated feed supplemented with FUMzyme (15 U/kg; FB+FUMzyme) for 14 days ad libitum. Addition of FUMzyme resulted in significantly decreased levels of FB1 in excreta, whereas HFB1 concentrations were significantly increased. Compared to the FB group (0.24 +/- 0.02), the mean serum sphinganine-to-sphingosine (Sa/So) ratio was significantly reduced in the FB+FUMzyme group (0.19 +/- 0.02), thus resembling values of the CON group (0.16 +/- 0.02). Similarly, exposure of piglets (n = 10) to 2 mg/kg FB1+FB2 for 42 days caused significantly elevated serum Sa/So ratios (0.39 +/- 0.15) compared to the CON group (0.14 +/- 0.01). Supplementation with FUMzyme (60 U/kg) resulted in gastrointestinal degradation of FB1 and unaffected Sa/So ratios (0.16 +/- 0.02). Thus, the carboxylesterase FumD represents an effective strategy to detoxify FB1 in the digestive tract of turkeys and pigs.
Autor*innen der BOKU Wien:
Schwartz-Zimmermann Heidi Elisabeth
BOKU Gendermonitor:


Find related publications in this database (Keywords)
toxicokinetics
biotransformation
detoxification
biomarker
Fusarium toxins
metabolism
swine
poultry
feed additive


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