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Gewählte Publikation:

Aryan, A; Musetti, R; Riedle-Bauer, M; Brader, G.
(2016): Phytoplasma Transmission by Heterologous Grafting Influences Viability of the Scion and Results in Early Symptom Development in Periwinkle Rootstock
J PHYTOPATHOL. 2016; 164(9): 631-640. FullText FullText_BOKU

Abstract:
The stolbur phytoplasma xxxCandidatus Phytoplasma solanixxx is responsible for the grapevine disease xxxbois noirxxx affecting a number of wine-growing areas in Europe. Transmission of stolbur phytoplasma to different laboratory hosts can be difficult due to the requirement of transmitting insect vectors or parasite plants. Here, heterologous grafting was used as an alternative technique for transmission of common and strongly symptomatic stolbur genotypes CPsM4_At1 and CPsM4_At6 of xxxCa. P. solanixxx to experimental host plants such as Catharanthus roseus and tomato making phytoplasma strains more accessible for molecular and experimental investigations in different plant species. Transmission was confirmed by quantitative PCR, microscopy and nested PCR followed by marker gene sequencing. In our study, the transmission of different genotypes of xxxCa. P. solanixxx resulted in distinguishable symptom development in the laboratory host C. roseus. Symptom development in grafted rootstock was observed three to 7 weeks after heterologous grafting. Survival of the graft unit was influenced by the presence of xxxCa. P. solanixxx in the scions and was clearly reduced in phytoplasma free scion - rootstock combinations.
Autor*innen der BOKU Wien:
Aryan Amal
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Candidatus Phytoplasma solani
grafting compatibility
grapevine
periwinkle
stolbur
tomato


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