BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Feichtinger, J; Hernandez, I; Fischer, C; Hanscho, M; Auer, N; Hackl, M; Jadhav, V; Baumann, M; Krempl, PM; Schmidl, C; Farlik, M; Schuster, M; Merkel, A; Sommer, A; Heath, S; Rico, D; Bock, C; Thallinger, GG; Borth, N.
(2016): Comprehensive Genome and Epigenome Characterization of CHO Cells in Response to Evolutionary Pressures and Over Time
BIOTECHNOL BIOENG. 2016; 113(10): 2241-2253. FullText FullText_BOKU

Abstract:
The most striking characteristic of CHO cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability. To investigate the relative contribution of genomic and epigenetic modifications towards phenotype evolution, comprehensive genome and epigenome data are presented for six related CHO cell lines, both in response to perturbations (different culture conditions and media as well as selection of a specific phenotype with increased transient productivity) and in steady state (prolonged time in culture under constant conditions). Clear transitions were observed in DNA-methylation patterns upon each perturbation, while few changes occurred over time under constant conditions. Only minor DNA-methylation changes were observed between exponential and stationary growth phase; however, throughout a batch culture the histone modification pattern underwent continuous adaptation. Variation in genome sequence between the six cell lines on the level of SNPs, InDels, and structural variants is high, both upon perturbation and under constant conditions over time. The here presented comprehensive resource may open the door to improved control and manipulation of gene expression during industrial bioprocesses based on epigenetic mechanisms. (C) 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
Autor*innen der BOKU Wien:
Auer Norbert
Baumann Martina
Borth Nicole
Hackl Matthias
Hanscho Michael
Hernandez-Lopez Inmaculada
Jadhav Vaibhav
Krempl Peter
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Chinese hamster ovary cells
epigenome
genome variation
DNA-methylation
histone modifications


Altmetric:
© BOKU Wien Impressum