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Gewählte Publikation:

Leitner, C., Haltrich, D., Nidetzky, B., Prillinger, H., Kulbe, K. D..
(1998): Production of a novel pyranose-2-oxidase by the basidiomycete Trametes multicolor.
Appl. Biochem. Biotechnol., 70-72, 237-248

Abstract:
During a screening for the enzyme pyranose 2-oxidase (P2O) which has a great potential as a biocatalyst for carbohydrate transformations, Trametes multicolor was identified as a promising, not-yet-described producer of this particular enzyme activity. Furthermore, it was found in this screening that the enzyme frequently occurs in basidiomycetes. Intracellular P2O was produced in a growth-associated manner by T. multicolor during growth on various substrates, including mono-, oligo-, and polysaccharides. Highest levels of this enzyme activity were formed when lactose or whey were used as substrates. Peptones from casein and other casein hydrolysates were found to be the most favorable nitrogen sources for the formation of P2O. By applying an appropriate feeding strategy for the substrate lactose, which ensured an elevated concentration of the carbon source during the entire cultivation, levels of P2O activity obtained in laboratory fermentations, as well as the productivity of these bioprocess experiments, could be enhanced more than 2.5-fold.
Autor*innen der BOKU Wien:
Haltrich Dietmar
Kulbe Klaus Dieter
Prillinger Hansjörg
Find related publications in this database (using NML MeSH Indexing)
Basidiomycota/metabolism*;Carbohydrate Dehydrogenases/biosynthesis*;Culture Media;Fermentation;Lactose/chemistry;Milk Proteins/chemistry;Monosaccharides/chemistry;Oligosaccharides/chemistry;Peptones/chemistry;Polysaccharides/chemistry;

Find related publications in this database (Keywords)
Trametes multicolor
pyranose 2-oxidase
screening
culture medium development


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