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Gewählte Publikation:

Rasche, F; Lueders, T; Schloter, M; Schaefer, S; Buegger, F; Gattinger, A; Hood-Nowotny, RC; Sessitsch, A.
(2009): DNA-based stable isotope probing enables the identification of active bacterial endophytes in potatoes
NEW PHYTOL. 2009; 181(4): 802-807. FullText FullText_BOKU

A (13)CO(2) (99 atom-%, 350 ppm) incubation experiment was performed to identify active bacterial endophytes in two cultivars of Solanum tuberosum, cultivars DesirEe and Merkur. We showed that after the assimilation and photosynthetic transformation of (13)CO(2) into (13)C-labeled metabolites by the plant, the most directly active, cultivar-specific heterotrophic endophytic bacteria that consume these labeled metabolites can be identified by DNA stable isotope probing (DNA-SIP). Density-resolved DNA fractions obtained from SIP were subjected to 16S rRNA gene-based community analysis using terminal restriction fragment length polymorphism analysis and sequencing of generated gene libraries. Community profiling revealed community compositions that were dominated by plant chloroplast and mitochondrial 16S rRNA genes for the xxxlightxxx fractions of (13)CO(2)-incubated potato cultivars and of potato cultivars not incubated with (13)CO(2). In the xxxheavyxxx fractions of the (13)CO(2)-incubated endophyte DNA, a bacterial 492-bp terminal restriction fragment became abundant, which could be clearly identified as Acinetobacter and Acidovorax spp. in cultivars Merkur and DesirEe, respectively, indicating cultivar-dependent distinctions in (13)C-label flow. These two species represent two common potato endophytes with known plant-beneficial activities. The approach demonstrated the successful detection of active bacterial endophytes in potato. DNA-SIP therefore offers new opportunities for exploring the complex nature of plant-microbe interactions and plant-dependent microbial metabolisms within the endosphere. New Phytologist (2009) 181: 802-807doi: 10.1111/j.1469-8137.2008.02744.x.
Autor*innen der BOKU Wien:
Hood-Nowotny Rebecca

Find related publications in this database (Keywords)
(13)C-DNA stable isotope probing
16S rRNA gene-based community analysis
active bacterial endophytes
plant-microbe interaction
Solanum tuberosum (potato)

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