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Gewählte Publikation:

Ruprecht, C; Dallabernardina, P; Smith, PJ; Urbanowicz, BR; Pfrengle, F.
(2018): Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls
CHEMBIOCHEM. 2018; 19(8): 793-798. FullText FullText_BOKU

Abstract:
The plant cell wall is a cellular exoskeleton consisting predominantly of a complex polysaccharide network that defines the shape of cells. During growth, this network can be loosened through the action of xyloglucan endotransglycosylases (XETs), glycoside hydrolases that cut and paste xyloglucan polysaccharides through a transglycosylation process. We have analyzed cohorts of XETs in different plant species to evaluate the substrate specificities of xyloglucan acceptors by using a set of synthetic oligosaccharides obtained by automated glycan assembly. The ability of XETs to incorporate the oligosaccharides into polysaccharides printed as microarrays and into stem sections of Arabidopsis thaliana, beans, and peas was assessed. We found that single xylose substitutions are sufficient for transfer, and xylosylation of the terminal glucose residue is not required by XETs, independent of plant species. To obtain information on the potential xylosylation pattern of the natural acceptor of XETs, that is, the nonreducing end of xyloglucan, we further tested the activity of xyloglucan xylosyl transferase (XXT) 2 on the synthetic xyloglucan oligosaccharides. These data shed light on inconsistencies between previous studies towards determining the acceptor substrate specificities of XETs and have important implications for further understanding plant cell wall polysaccharide synthesis and remodeling.
Autor*innen der BOKU Wien:
Pfrengle Fabian
Ruprecht Colin
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Cell Wall/metabolism*
Chromatography, High Pressure Liquid
Glycosyltransferases/metabolism*
Oligosaccharides/metabolism*
Plants/classification
Plants/metabolism
Species Specificity

Find related publications in this database (Keywords)
carbohydrates
endotransglycosylases
fluorescent probes
plant cell walls
polysaccharide glycan arrays
xylosyltransferases


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