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Gewählte Publikation:

Beer, B; Bartolome, MJ; Berndorfer, L; Bochmann, G; Guebitz, GM; Nyanhongo, GS.
(2020): Controlled enzymatic hydrolysis and synthesis of lignin cross-linked chitosan functional hydrogels
INT J BIOL MACROMOL. 2020; 161: 1440-1446. FullText FullText_BOKU

Abstract:
This study presents a novel fully enzymatic process for the controlled depolymerisation of fungal and shrimp chitosan, and their subsequent use in the synthesis of lignin cross-linked chitosan (CTS) hydrogels. Cellobiosehydrolase (CBH) was used to depolymerize CTS resulting in decrease in average molecular weight (Mw) of shrimp CTS from 140 kDa and degree of deacetylation (DD %) from 91.3% to an average MW of 15 kDa and 16% DD. Similarly, fungal chitosan average molecular weight decreased from 92 kDa and the degree of deacetylation (DD) of 48.3% to 12 kDa and a DD of 13%. The depolymerized CTS were completely soluble in water and miscible with lignosulfonates without encountering the usual problem of formation of flocs. Introduction of lactase into a lignosulfonate-chitosan mixture resulted in the oxidation and generation of lignin reactive phenoxyl radicals that cross-linked with CTS-N H-2 reactive groups resulting in the increase of Mw from 20 kDa to >500 kDa and viscosity from 20 mPa to >500 mPa. This resulted in the formation of stable lignin-cross-linked hydrogels with elongation at break of 111% and tensile strength of 7 mPa. The produced functional hydrogels have potential application in food and biomedical industries as e.g. as oxygen barriers in packaging or as functional wound dressing or tissue engineering platforms. (C) 2020 Elsevier B.V. All rights reserved.
Autor*innen der BOKU Wien:
Beer Bianca
Berndorfer Lisa
Bochmann Günther
Gübitz Georg
BOKU Gendermonitor:


Find related publications in this database (Keywords)
lignin cross-linked chitosan hydrogels
Laccase
Cellobiohydrolase
Lignin
Chitosan


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