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Gewählte Publikation:

Brechtel, E; Matuschek, M; Hellberg, A; Egelseer, EM; Schmid, R; Bahl, H.
(1999): Cell wall of Thermoanaerobacterium thermosulfurigenes EM1: isolation of its components and attachment of the xylanase XynA.
Arch Microbiol. 1999; 171(3):159-165

Abstract:
Thermoanaerobacterium thermosulfurigenes EMI has a gram-positive type cell wall completely covered by a surface layer (S-layer) with hexagonal lattice symmetry. The components of the cell envelope were isolated, and the S-layer protein was purified and characterized. S-layer monomers assembled in vitro into sheets with the same hexagonal symmetry as in vivo. Monosaccharide analysis revealed that the S-layer is associated with fucose, rhamnose, mannosamine, glucosamine, galactose, and glucose. The N-terminal 31 amino acid residues of the S-layer protein showed significant similarity to SLH (S-layer homology) domains found in S-layer proteins of different bacteria and in the exocellular enzymes pullulanase, polygalacturonate hydrolase, and xylanase of T. thermosulfurigenes EM1. The xylanase from T. thermosulfurigenes EMI was copurified with the Slayer protein during isolation of cell wall components. Since SLH domains of some structural proteins have been shown to anchor these proteins noncovalently to the cell envelope, we propose a common anchoring mechanism for the S-layer protein and exocellular enzymes via their SLH domains in the peptidoglycan-containing layer of T. thermosulfurigenes EM1.
Autor*innen der BOKU Wien:
Egelseer Eva Maria
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Bacteria, Anaerobic - chemistry
Bacterial Proteins - chemistry
Cell Wall - chemistry
Chromatography, Ion Exchange - chemistry
Electrophoresis, Polyacrylamide Gel - chemistry
Membrane Proteins - chemistry
Microscopy, Electron - chemistry
Molecular Sequence Data - chemistry
Monosaccharides - analysis
Xylan Endo-1,3-beta-Xylosidase - analysis
Xylosidases - isolation & purification

Find related publications in this database (Keywords)
S-layer
thermophile
exocellular proteins
cell surface


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