BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Breuer, S; Marzban, G; Cserjan-Puschman, M; Dürrschmid, E; Bayer, K.
(1998):
Off-line quantitative monitoring of plasmid copy number in bacterial fermentation by capillary electrophoresis.
Electrophoresis. 1998; 19(14):2474-2478
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- Abstract:
- Capillary electrophoresis (CE) is an effective instrumental alternative to conventional slab gel electrophoresis in the determination of plasmid copy number during recombinant protein formation processes. This analytical setup provides efficient separation of different species of linearized plasmid molecules and quantification by UV detection. Both fused silica and gel-filled capillaries are assessed with respect to peak resolution and reproducibility. The application of coated capillaries eliminates the electroosmatic flow to a large extent, resulting in excellent separation of DNA fragments. The application of UV detection enables the analysis of linearized plasmid DNA with a conventional laboratory CE device. All investigated plasmids show good peak resolution due to their significant differences in molecular size, which is essential for sufficient separation of individual DNA molecules.
- Autor*innen der BOKU Wien:
- Bayer Karl
- Marzban Gorji
- BOKU Gendermonitor:
- Find related publications in this database (using NML MeSH Indexing)
- Bacteria - metabolism
- Bacteriophage lambda - genetics
- Buffers -
- DNA, Viral - isolation & purification
- Deoxyribonuclease HindIII - metabolism
- Electrophoresis, Capillary - methods
- Escherichia coli - genetics
- Fermentation -
- Gels -
- Molecular Weight -
- Plasmids - analysis
- Recombinant Proteins - biosynthesis
- Silicon Dioxide -
- Find related publications in this database (Keywords)
- plasmid copy number
- DNA separation
- capillary electrophoresis
- liquid polymer
- bacterial fermentation
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