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Gewählte Publikation:

Strutzenberger, K; Borth, N; Kunert, R; Steinfellner, W; Katinger, H.
(1999): Changes during subclone development and ageing of human antibody-producing recombinant CHO cells.
J Biotechnol. 1999; 69(2-3):215-226

Some of the problems encountered with human or human-mouse heterohybridomas, such as low growth rates and high serum requirements, have led to the increased use of recombinant cell lines for production of human antibodies. To evaluate the suitability of such alternative cell lines for the production of human antibodies we have analysed several subclones with differing specific production rates of a recombinant CHO cell line. Gene copy number and site of chromosomal integration for the light and heavy chain and the dhfr gene were determined by in-situ hybridisation. Specific mRNA content was analysed by Northern blot. In addition the intracellular content in light and heavy chain was measured by flow cytometry and the specific secretion rates were determined. The stability of gene expression was followed in the highest producing subclone for over a year. As previously seen in heterohybridoma cells a high expression rate of light chain is beneficial in speeding up secretion rates of whole antibody. When grown in the presence of G418 and methotrexate the amplified gene copies in the genome of recombinant CHO cells were stable over more than 100 passages. However, the expression of light chain, and with it the secretion rate, decreased with time. The low intracellular concentration of light chain resulted in accumulation of heavy chain in the endoplasmic reticulum due to retention by chaperones. The specific secretion rate decreased by 50% after 100 passages. When no G418 or methotrexate were present 75% of the gene copies were lost after 100 passages. (C) 1999 Elsevier Science B.V. All rights reserved.
Autor*innen der BOKU Wien:
Borth Nicole
Katinger Hermann
Kunert Renate
Find related publications in this database (using NML MeSH Indexing)
Animals -
CHO Cells -
Cell Aging -
Clone Cells - immunology
Cricetinae - immunology
Enzyme-Linked Immunosorbent Assay - immunology
Flow Cytometry - immunology
Fluorescent Antibody Technique - immunology
Gene Expression - immunology
Gentamicins - pharmacology
Humans - pharmacology
Hybridomas - pharmacology
Immunoglobulin G - biosynthesis
Immunoglobulin Heavy Chains - biosynthesis
Immunoglobulin Light Chains - biosynthesis
In Situ Hybridization - biosynthesis
Methotrexate - pharmacology
Recombinant Proteins - biosynthesis
Tetrahydrofolate Dehydrogenase - genetics
Transfection - genetics

Find related publications in this database (Keywords)
antibody production
recombinant Chinese hamster ovary cells
light and heavy chain

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