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Wilson, IB; .
(2001): Identification of a cDNA encoding a plant Lewis-type alpha1,4-fucosyltransferase.
Glycoconj J. 2001; 18(6):439-447

Abstract:: Recently, it has been found that plants, including tomato (Lycopersicon esculentum), express the Lewis-a epitope, Galbeta1,3(Fucalpha1,4)GlcNAc, on some N-glycans. By searching the EST database, it was possible to identify a tomato cDNA encoding a protein, designated FucTC, of 413 amino acids with homology to plant and mammalian alpha1,3/4-fucosyltransferases. The cDNA was expressed in Pichia pastoris and the recombinant enzyme was found to transfer fucose from GDP-Fuc (K-m 16 muM) to lacto-N-tetraose (Galbeta1,3GlcNAcbeta1,3Galbeta1,4Glc; K-m 80 muM) as well as to beta1,3- and beta1,4-galactosylated N-glycans. It is concluded that FucTC is responsible for the biosynthesis of Lewis-a on N-glycans in tomato.
Autor*innen der BOKU Wien:: Wilson Iain B.H.
Find related publications in this database (using NML MeSH Indexing): Amino Acid Sequence;Animals;Base Sequence;Chromatography, High Pressure Liquid;Cloning, Molecular;Fucosyltransferases/chemistry;Fucosyltransferases/genetics*;Humans;Lycopersicon esculentum/enzymology*;Lycopersicon esculentum/genetics*;Molecular Sequence Data;Phylogeny;Sequence Homology, Amino Acid;Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Find related publications in this database (Keywords): fucosyltransferase; tomato; Lewis-a