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Gewählte Publikation:

Beckedorf, AI; Schäffer, C; Messner, P; Peter-Katalinic, J.
(2002): Mapping and sequencing of cardiolipins from Geobacillus stearothermophilus NRS 2004/3a by positive and negative ion nanoESI-QTOF-MS and MS/MS.
J Mass Spectrom. 2002; 37(10):1086-1094 FullText FullText_BOKU

Abstract:
In the course of systematic studies on surface layer (S-layer) glycoproteins of bacilli, the chloroform/methanol extract from whole cells of Geobacillus stearothermophilus NRS 2004/3a has been submitted to MS analysis. Glucosylated cardiolipins were found as minor components of the total lipid and phospholipid mixture by de novo identification. After purification of the crude extract using a combined column chromatography/2D TLC protocol, structural investigations of components in the lipid fraction by high resolution ESI-QTOF MS analysis provided evidence about homologous molecules attributable to the cardiolipin species containing a glycosylated backbone, and about a diversity of ester-linked fatty acid substituents. In comparative studies by positive and negative ion nanoESI-QTOF-CID-MS, maps of cardiolipin molecular ions were obtained, followed by MS/MS of the most abundant species, to provide structural details of D-glucopyranosylcardiolipin and the fatty acid substituent patterns. Experiments of the parent ion scan type revealed the presence of fatty acid moieties as isobaric combinations, represented in single molecular ion species. Copyright (C) 2002 John Wiley Sons, Ltd.
Autor/innen der BOKU Wien:
Messner Paul
Schäffer Christina
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Cardiolipins - analysis
Fatty Acids - analysis
Gram-Positive Bacteria - chemistry
Lipids - analysis
Models, Molecular -
Monosaccharides - analysis
Spectrometry, Mass, Electrospray Ionization -

Find related publications in this database (Keywords)
positive and negative ion nanoESI-QTOF MS/MS
negative ion mode precursor ion scan
mapping and sequencing of glycophospholipids
glucocardiolipins
Gram-positive organism


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