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Gewählte Publikation:

Amer, H; Hofinger, A; Kosma, P.
(2003): Synthesis of neoglycoproteins containing O-methylated trisaccharides related to excretory/secretory antigens of Toxocara larvae.
Carbohydr Res. 2003; 338(1):35-45

Abstract:
The disaccharides allyl beta-D-galactopyranosyl-(1 --> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 10a and 10b and the trisaccharides allyl 2-O-methyl-alpha-L-fucopyranosyl-(1 --> 2)-beta-D-galactopyranosyl-(1--> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 18a and 18b have been prepared using stepwise assembly of the sugar units. The glycosidic linkages were formed employing the trichloroacetimidate procedure for the attachment of the galactopyranosyl residue and N-iodosuccinimide/triflic acid activation of an ethyl 1-thiofucopyranoside donor for fucosylation. Deprotection furnished the allyl glycosides which were converted into cysteamine-spacered ligands, activated with thiophosgene and subsequently linked to bovine serum albumin. The neoglycoproteins serve as immunoreagents to determine epitope specificities of monoclonal antibodies directed against highly immunogenic O-glycans located at the surface of Toxocara larvae. (C) 2002 Elsevier Science Ltd. All rights reserved.
Autor*innen der BOKU Wien:
Hofinger-Horvath Andreas
Kosma Paul
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Antigens, Helminth - chemistry
Cattle -
Glycoproteins - chemical synthesis
Helminth Proteins - chemistry
Larva - immunology
Serum Albumin, Bovine - chemistry
Toxocara - immunology
Trisaccharides - chemical synthesis

Find related publications in this database (Keywords)
neoglycoprotein
Toxocara
antigen
O-glycan
fucose


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