BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Fuchs, E; Binder, EM; Heidler, D; Krska, R.
(2002):
Structural characterization of metabolites after the microbial degradation of type A trichothecenes by the bacterial strain BBSH 797.
Food Addit Contam. 2002; 19(4):379-386
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- Abstract:
- Contamination of feed with trichothecenes, a group of Fusarium mycotoxins, leads to losses in performance due to their immunosupressive effects and the negative effect on the gastrointestinal system in animal production. A possible way of detoxification is microbial degradation, which was the focus of this study. A bacterial strain-BBSH 797-which can degrade some mycotoxins of the trichothecene group, has already been isolated. It transforms deoxynivalenol (DON) into its metabolite DOM-1, the non-toxic deepoxide of DON. Analogous to the microbial degradation of DON, the transformation of six different type A trichothecenes was observed. The metabolites appearing were characterized by GC-MS after derivatization with TRI-SIL(R)TBT. Two metabolites were additionally identified by liquid chromatography-mass spectrometry with particle beam interface (LC-PB-MS) with electron impact (EI)-ionization mode. The major finding was that scirpentriol was completely transformed into its non-toxic metabolite deepoxy scirpentriol, while the mycotoxin T-2 triol underwent a more complicated metabolism. According to the study, T-2-triol was degraded into its non-toxic deepoxy form and into T-2 tetraol, which was then further metabolized to deepoxy T-2 tetraol. GC-MS after derivatization with TRI-SIL(R)TBT was suitable for the structural characterization of trichothecenes and their degradation products. Besides the mass spectra of already known degradation products, spectra of new metabolites could be recorded by LC-PB-MS.
- Autor*innen der BOKU Wien:
- Krska Rudolf
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Chromatography, Liquid - methods
- Food Contamination - methods
- Gas Chromatography-Mass Spectrometry - methods
- Gram-Positive Asporogenous Rods, Irregular - metabolism
- Metabolic Detoxication, Drug - metabolism
- T-2 Toxin - analogs & derivatives
- Trichothecenes - pharmacokinetics
- Find related publications in this database (Keywords)
- trichothecenes
- detoxification
- mycotoxin
- mass spectrometry
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