BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Léonard, R., Kolarich, D., Paschinger, K., Altmann, F., Wilson, I.B.H..
(2004):
A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons
Plant Mol. Biol., 55, 631-644
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- Abstract:
- The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core alpha1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and beta1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.
- Autor*innen der BOKU Wien:
- Altmann Friedrich
- Leonard Renaud
- Paschinger Katharina
- Wilson Iain B.H.
- Find related publications in this database (Keywords)
- fucosyltransferase
- glycan
- glucosyltransferase
- monocotyledon
- N-acetylglucosaminyltransferase
- oligosaccharide
- rice
- xylosyltransferase
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