BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Léonard, R., Kolarich, D., Paschinger, K., Altmann, F., Wilson, I.B.H..
(2004): A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons
Plant Mol. Biol., 55, 631-644 FullText FullText_BOKU

The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC, core alpha1,3-fucosyltransferase (FucTA; EC and beta1,2-xylosyltransferase (EC and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.
Autor*innen der BOKU Wien:
Altmann Friedrich
Leonard Renaud
Paschinger Katharina
Wilson Iain B.H.

Find related publications in this database (Keywords)

© BOKU Wien Impressum