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Gewählte Publikation:

Sessitsch, A., S. Gyamfi, D. Tscherko, M.H. Gerzabek and E. Kandeler.
(2004): Activity of microorganisms in the rhizosphere of herbicide treated and untreated transgenic glufosinate-tolerant and wildtype oilseed rape grown in containment
Plant and Soil, 266, 105-116 FullText FullText_BOKU

An experiment was carried out with plants grown in containment in order to determine potential effects on metabolically active microbial populations as well as on soil enzyme activities in the rhizosphere of genetically modified Basta-tolerant oilseed rape. Transgenic as well as isogenic wild-type plants were grown in combination with the associated herbicide Basta (active ingredient glufosinate) or the herbicide Butisan S (active ingredient metazachlor), respectively. In control treatments, weeds were mechanically removed. Rhizosphere soil was sampled at the early and late flowering stage as well as at senescence. RNA was isolated and 16S rRNA was amplified by reverse transcription and PCR. Amplicons were subjected to denaturating gradient gel electrophoresis in order to generate community fingerprints of metabolically highly active bacteria. Additionally, RNA was hybridized with group-specific probes. Furthermore, bacterial biomass and activities of invertase, alkaline phosphatase, urease and arylsulfatase were determined. Results showed that oilseed rape rhizosphere bacteria were affected by the genetic modification as well as by the herbicide application, however, these effects were minor compared to the influence of the plant growth stage. At senescence, invertase, urease and alkaline phosphatase activities were significantly enhanced in the rhizospheres of transgenic plants as compared to wild-type plants propably due to an altered root exudation in comparison to the wildtype plant. Dot blot hybridizations indicated altered activities and/or abundances of various phylogenetic groups at all sampling times. The transformation process may have unintentionally altered the physiology of plants (e.g. root exudation) leading to changes in rhizosphere community structures and bacterial activities.
Autor*innen der BOKU Wien:
Gerzabek Martin

Find related publications in this database (Keywords)
Butisan S
dot-blot hybridization
enzyme activities
pat gene
16S rRNA

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