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Gewählte Publikation:

Leitsch, D; Radauer, C; Paschinger, K; Wilson, IB; Breiteneder, H; Scheiner, O; Duchêne, M.
(2005): Entamoeba histolytica: analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis.
Exp Parasitol. 2005; 110(3):191-195 FullText FullText_BOKU

The Entamoeba histolytica genome project carried out at TIGR and the Sanger Institute has produced a near-complete set of deduced open reading frame sequences. These data provide strong support for the identification of signals from proteomic analyses such as two-dimensional electrophoresis by protein sequencing and/or mass spectrometric methods. To carry out an initial investigation of the E histolytica proteome, appropriate sample preparation and silver staining protocols were adapted. After preparation of protein extracts from E histolytica HM-1:1MSS trophozoites, solubilized proteins were separated in the first dimension in IPG (immobilized pH gradient) strips depending on their pI and subsequently in the second dimension according to their molecular weight by SDS-polyacrylamide gel electrophoresis. Of the more than 1500 protein spots visualized, several landmark spots were isolated from the gels and identified by either tryptic cleavage and subsequent MALDI-TOF mass spectrometry or by protein sequencing. (c) 2005 Elsevier Inc. All rights reserved.
Autor*innen der BOKU Wien:
Paschinger Katharina
Wilson Iain B.H.
Find related publications in this database (using NML MeSH Indexing)
Animals -
Electrophoresis, Gel, Two-Dimensional -
Entamoeba histolytica - chemistry
Hydrogen-Ion Concentration - chemistry
Proteome - analysis
Protozoan Proteins - analysis
Sequence Analysis, Protein - analysis
Silver Staining - analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - analysis

Find related publications in this database (Keywords)
Entamoeba histolytica
two-dimensional gel electrophoresis
mass spectrometry

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