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Gewählte Publikation:

Bohrn, R., Potthast, A., Rosenau, T., Sixta, H., Kosma, P..
(2005): Synthesis and testing of a novel fluorescence label for carboxyls in carbohydrates and cellulosics
Synlett, 20, 3087-3090 FullText FullText_BOKU

Abstract:
A carboxyl-selective fluorescence label, 9H-fluoren-2-yl-diazomethane (FDAM, 6) for the detection of carboxyl groups specifically in polysaccharides and low-molecular weight carbohydrates has been developed. Attached in a pre-column derivatization step, the label allows detection of minute amounts of oxidized functionalities in oligosaccharides and cellulosics under the relatively harsh conditions of GPC separation in the DMAc/LiCl eluant and without any fluorescence interference with light scattering (MALLS) detection, so that carboxyl profiles of polysaccharides were obtained for the first time. Synthetic carbohydrate model compounds (1-3) were used to optimize the labeling reaction.
Autor*innen der BOKU Wien:
Kosma Paul
Potthast Antje
Rosenau Thomas
BOKU Gendermonitor:


Find related publications in this database (Keywords)
carboxyls
polysaccharides
uronic acids
fluorescence labeling
carbohydrates


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