BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Gambino, G; Gribaudo, I; Leopold, S; Schartl, A; Laimer, M.
(2005):
Molecular characterization of grapevine plants transformed with GFLV resistance genes: I.
Plant Cell Rep. 2005; 24(11):655-662
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- Abstract:
- The Grapevine FanLeaf Virus-Coat Protein ( GFLV CP) gene was inserted through Agrobacterium-mediated transformation in Vitis vinifera 'Nebbiolo', 'Lumassina' and 'Blaufrankisch'. Two plasmids were used: pGA-CP+ (full-length GFLV CP gene with an introduced start codon) and pGA-AS ( same gene in antisense orientation). Forty-three transgenic lines were regenerated. As several lines in Southern blots share same hybridization patterns, eight independent line groups resulted for 'Nebbiolo', one for 'Lumassina', and two for 'Blaufrankisch'. Inserted T-DNA copies ranged from one to three; one line probably contains an incomplete copy of T-DNA. Except for one 'Nebbiolo' line, no evidence for methylation of the transgene at cytosine residues was found by Southern analyses. Specific mRNA was present at variable expression levels; some lines accumulated the coat protein while in others the protein was not detectable by ELISA.
- Autor*innen der BOKU Wien:
- Laimer Margit
- Find related publications in this database (using NML MeSH Indexing)
- Blotting, Southern -
- DNA, Plant -
- Gene Expression -
- Immunity, Innate - genetics
- Plant Viruses - immunology
- Plants - anatomy & histology
- Plants, Genetically Modified - anatomy & histology
- RNA, Messenger - genetics
- Rhizobium - genetics
- Find related publications in this database (Keywords)
- nepovirus
- pathogen-derived resistance
- somatic embryogenesis
- grapevine
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