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Gewählte Publikation:

Braun, E; Wagner, A; Fürnschlief, E; Katinger, H; Vorauer-Uhl, K.
(2006): Experimental design for in vitro skin penetration study of liposomal superoxide dismutase.
J Pharm Biomed Anal. 2006; 40(5):1187-1197 FullText FullText_BOKU

Abstract:
A computer-based technique using a 2((5-2)) fractional factorial design was applied for screening the factors affecting the penetration effectiveness of liposomal recombinant human-Cu/Zn-superoxide dismutase. Unilamellar liposomes, containing recombinant rh-Cu/Zn-SOD in the aqueous core, are aimed at enhancing the penetration of the drug applied topically. Factors that mainly influence the chemical and physical characteristics of liposomes such as charge, molar content of cholesterol, size, surfactant and lipid were evaluated at two levels. In vitro skin penetration studies with pigskin were carried out in Franz-type diffusion cells over a period of 4 and 8 h. The response variables, namely the amounts of rh-Cu/Zn-SOD penetrated into the different skin layers, were analyzed by ELISA (enzyme linked immunosorbent assay). Analysis of variance showed that the size and the cholesterol content of liposomes as well as the duration of the penetration studies have a statistically significant influence on the amount of protein found in deeper skin layers. (c) 2005 Elsevier B.V. All rights reserved.
Autor*innen der BOKU Wien:
Katinger Hermann
Vorauer-Uhl Karola
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
1,2-Dipalmitoylphosphatidylcholine -
Algorithms -
Animals -
Cholesterol - chemistry
Diffusion Chambers, Culture - chemistry
Drug Carriers - chemistry
Enzyme-Linked Immunosorbent Assay - chemistry
Factor Analysis, Statistical - chemistry
Female - chemistry
Liposomes - chemistry
Male - chemistry
Particle Size - chemistry
Proteins - chemistry
Recombinant Proteins - administration & dosage
Skin Absorption - drug effects
Superoxide Dismutase - administration & dosage
Swine - administration & dosage

Find related publications in this database (Keywords)
experimental design
in vitro skin penetration study
liposome
superoxide dismutase
Rh-Cu/Zn-SOD
biotechnology
drug delivery


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