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Gewählte Publikation:

Rhomberg, S; Fuchsluger, C; Rendic, D; Paschinger, K; Jantsch, V; Kosma, P; Wilson, IB.
(2006): Reconstitution in vitro of the GDP-fucose biosynthetic pathways of Caenorhabditis elegans and Drosophila melanogaster.
FEBS J. 2006; 273(10):2244-2256 FullText FullText_BOKU

The deoxyhexose sugar fucose has an important fine-tuning role in regulating the functions of glycoconjugates in disease and development in mammals. The two genetic model organisms Caenorhabditis elegans and Drosophila melanogaster also express a range of fucosylated glycans, and the nematode particularly has a number of novel forms. For the synthesis of such glycans, the formation of GDP-fucose, which is generated from GDP-mannose in three steps catalysed by two enzymes, is required. By homology we have identified and cloned cDNAs encoding these two proteins, GDP-mannose dehydratase (GMD; EC and GDP-keto-6-deoxymannose 3,5-epimerase/4-reductase (GER or FX protein; EC, from both Caenorhabditis and Drosophila. Whereas the nematode has two genes encoding forms of GMD (gmd-1 and gmd-2) and one GER-encoding gene (ger-1), the insect has, like mammalian species, only one homologue of each (gmd and gmer). This compares to the presence of two forms of both enzymes in Arabidopsis thaliana. All corresponding cDNAs from Caenorhabditis and Drosophila, as well as the previously uncharacterized Arabidopsis GER2, were separately expressed, and the encoded proteins found to have the predicted activity. The biochemical characterization of these enzymes is complementary to strategies aimed at manipulating the expression of fucosylated glycans in these organisms.
Autor*innen der BOKU Wien:
Fuchsluger Christina
Kosma Paul
Paschinger Katharina
Rendic Dubravko
Wilson Iain B.H.
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Animals -
Arabidopsis - enzymology
Arabidopsis Proteins - genetics
Caenorhabditis elegans - enzymology
Caenorhabditis elegans Proteins - genetics
Carbohydrate Epimerases - genetics
Cloning, Molecular - genetics
DNA, Complementary - genetics
Drosophila Proteins - genetics
Drosophila melanogaster - enzymology
Gene Expression Regulation, Developmental - enzymology
Guanosine Diphosphate Fucose - chemistry
Guanosine Diphosphate Mannose - analogs & derivatives
Humans - analogs & derivatives
Hydro-Lyases - genetics
Molecular Sequence Data - genetics
Protein Isoforms - genetics
Sequence Alignment - genetics
Sequence Homology, Amino Acid - genetics
Species Specificity - genetics

Find related publications in this database (Keywords)
GDP-fucose biosynthesis
GDP-keto-6-deoxymannose 3
5-epimerase /4-reductase
GDP-mannose dehydratase

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