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Gewählte Publikation:

Paschinger, K; Hackl, M; Gutternigg, M; Kretschmer-Lubich, D; Stemmer, U; Jantsch, V; Lochnit, G; Wilson, IB.
(2006): A deletion in the golgi alpha-mannosidase II gene of Caenorhabditis elegans results in unexpected non-wild-type N-glycan structures.
J Biol Chem. 2006; 281(38):28265-28277 FullText FullText_BOKU

The processing of N-linked oligosaccharides by alpha-mannosidases in the endoplasmic reticulum and Golgi is a process conserved in plants and animals. After the transfer of a GlcNAc residue to Asn- bound Man(5)GlcNAc(2) by N-acetylglucosaminyl-transferase I, an alpha-mannosidase (EC removes one alpha 1,3- linked and one alpha 1,6- linked mannose residue. In this study, we have identified the relevant alpha-mannosidase II gene (aman- 2; F58H1.1) from Caenorhabditis elegans and have detected its activity in both native and recombinant forms. For comparative studies, the two other cDNAs encoding class II mannosidases aman-1 (F55D10.1) and aman- 3 (F48C1.1) were cloned; the corresponding enzymes are, respectively, a putative lysosomal alpha-mannosidase and a Co(II)-activated alpha-mannosidase. The analysis of the N-glycan structures of an aman- 2 mutant strain demonstrates that the absence of alpha-mannosidase II activity results in a shift to structures not seen in wild-type worms (e. g. N-glycans with the composition Hex(5-7)HexNAc(2-3)Fuc(2)Me) and an accumulation of hybrid oligosaccharides. Paucimannosidic glycans are almost absent from aman-2 worms, indicative also of a general lack of alpha-mannosidase III activity. We hypothesize that there is a tremendous flexibility in the glycosylation pathway of C. elegans that does not impinge, under standard laboratory conditions, on the viability of worms with glycotypes very unlike the wild-type pattern.
Autor*innen der BOKU Wien:
Hackl Matthias
Paschinger Katharina
Wilson Iain B.H.
Find related publications in this database (using NML MeSH Indexing)
Animals -
Caenorhabditis elegans - enzymology
Glycosylation -
Golgi Apparatus - enzymology
Mutation -
Polysaccharides - biosynthesis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -
alpha-Mannosidase - genetics

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