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Gewählte Publikation:

Hofmann, J; Grundler, FM.
(2006): Females and males of root-parasitic cyst nematodes induce different symplasmic connections between their syncytial feeding cells and the phloem in Arabidopsis thaliana.
Plant Physiol Biochem. 2006; 44(5-6):430-433 FullText FullText_BOKU

Abstract:
Root syncytia induced by the beet cyst nematode Heterodera schachtii were thought to be symplasmically isolated. A recent study with mobile and immobile GFP constructs expressed in transgenic Arabidopsis plants under the control of pAtSUC2 showed that only mobile GFP could be detected in syncytia and suggested the existence of plasmodesmata between syncytia and the phloem. In the present study the existence of plasmodesmata between syncytia and the phloem is proven by grafting experiments. This technique rules out the possibility that GFP accumulation in syncytia is due to GFP expression in syncytia. Mobile GFP could be followed from transgenic scions carrying a pAtSUC2-gfp fusion construct via wild-type rootstocks into nematode-induced syncytia. While GFP could be detected in all syncytia associated to female nematodes, it was never observed in syncytia of male juveniles. As no GFP-mRNA could be detected in the rootstock we postulate that GFP as protein entered syncytia of females via plasmodesmata, while the protein was excluded from syncytia of male juveniles by plasmodesmata with a lower size exclusion limit. (c) 2006 Elsevier SAS. All rights reserved.
Autor*innen der BOKU Wien:
Grundler Florian
Hofmann Julia
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Arabidopsis - cytology
Female - cytology
Giant Cells - cytology
Green Fluorescent Proteins - biosynthesis
Male - biosynthesis
Membrane Transport Proteins - biosynthesis
Nematoda - physiology
Plant Proteins - biosynthesis
Plant Roots - cytology
Plants, Genetically Modified - cytology
Plasmodesmata - metabolism
Recombinant Fusion Proteins - biosynthesis

Find related publications in this database (Keywords)
plasmodesmata
nematode
phloem
syncytium
AtSUC2
grafting


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