BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Probst, OC; Ton, P; Svoboda, B; Gannon, A; Schuhmann, W; Wieser, J; Pohlmann, R; Mach, L.
(2006):
The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.
J Cell Sci. 2006; 119(Pt 23):4935-4943
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- Abstract:
- In mammalian cells, the mannose 6-phosphate receptor pathway accounts for the transport of most soluble acid hydrolases to lysosomes. It is believed that dissociation of mannose 6-phosphate receptors and their ligands is entirely driven by the acidic environment in endosomal compartments. Indeed, pH-perturbing substances such as ammonium chloride and monensin have been shown to inhibit lysosomal enzyme targeting in cells that express both known mannose 6-phosphate receptors. We now demonstrate that ammonium chloride and monensin exert modest effects on the intracellular retention of lysosomal hydrolases in murine cells that synthesize only the 46-kDa mannose 6-phosphate receptor. Neither ammonium chloride nor monensin induces changes to the subcellular localization of lysosomal hydrolases and the 46-kDa mannose 6-phosphate receptor in these cells. This suggests that endosomal dissociation of the receptor and its ligands still occurs in the presence of these agents. We conclude that the murine 46-kDa mannose 6-phosphate receptor has the capacity to deliver its cargo proteins to lysosomes even in the absence of endosomal acidification.
- Autor*innen der BOKU Wien:
- Mach Lukas
- BOKU Gendermonitor:
- Find related publications in this database (using NML MeSH Indexing)
- 3T3-L1 Cells -
- Animals -
- Cathepsin B - metabolism
- Cathepsin D - metabolism
- Cathepsins - metabolism
- Cells, Cultured - metabolism
- Cysteine Endopeptidases - metabolism
- Endosomes - chemistry
- Glycosylation - chemistry
- Humans - chemistry
- Hydrogen-Ion Concentration - chemistry
- Hydrolases - metabolism
- Ionophores - pharmacology
- Lysosomes - drug effects
- Mice - drug effects
- Mice, Knockout - drug effects
- Monensin - pharmacology
- NIH 3T3 Cells - pharmacology
- Protein Transport - drug effects
- Receptor, IGF Type 2 - genetics
- Signal Transduction - genetics
- Tissue Distribution - genetics
- Find related publications in this database (Keywords)
- mannose 6-phosphate
- lysosome
- trafficking
- cathepsin
- hydrolase
- biosynthesis
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