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Gewählte Publikation:

Steiner, K; Novotny, R; Patel, K; Vinogradov, E; Whitfield, C; Valvano, MA; Messner, P; Schäffer, C.
(2007): Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a.
J Bacteriol. 2007; 189(7):2590-2598 FullText FullText_BOKU

The glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-> 2)-alpha-L-Rhap-(1 -> 3)-beta-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 ->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-L-rhamnose residues, and a beta-D-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium.
Autor*innen der BOKU Wien:
Messner Paul
Schäffer Christina
Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Bacillaceae - genetics
Bacterial Proteins - chemistry
Carbohydrate Sequence -
Conserved Sequence -
Galactosyltransferases - genetics
Lipopolysaccharides - chemistry
Membrane Glycoproteins - metabolism
Molecular Sequence Data -
Plasmids -
Polysaccharides - biosynthesis
Protein Conformation -
Sequence Alignment -
Sequence Homology, Amino Acid -

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