BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Steiner, K; Novotny, R; Patel, K; Vinogradov, E; Whitfield, C; Valvano, MA; Messner, P; Schäffer, C.
(2007):
Functional characterization of the initiation enzyme of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus NRS 2004/3a.
J Bacteriol. 2007; 189(7):2590-2598
FullText
FullText_BOKU
- Abstract:
- The glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-> 2)-alpha-L-Rhap-(1 -> 3)-beta-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 ->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-L-rhamnose residues, and a beta-D-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium.
- Autor*innen der BOKU Wien:
- Messner Paul
- Schäffer Christina
- Find related publications in this database (using NML MeSH Indexing)
- Amino Acid Sequence -
- Bacillaceae - genetics
- Bacterial Proteins - chemistry
- Carbohydrate Sequence -
- Conserved Sequence -
- Galactosyltransferases - genetics
- Lipopolysaccharides - chemistry
- Membrane Glycoproteins - metabolism
- Molecular Sequence Data -
- Plasmids -
- Polysaccharides - biosynthesis
- Protein Conformation -
- Sequence Alignment -
- Sequence Homology, Amino Acid -
Altmetric: