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Gewählte Publikation:

Fortschegger, K; Wagner, B; Voglauer, R; Katinger, H; Sibilia, M; Grillari, J.
(2007): Early embryonic lethality of mice lacking the essential protein SNEV.
Mol Cell Biol. 2007; 27(8):3123-3130 FullText FullText_BOKU

Abstract:
SNEV (Prp19, Pso4, NMP200) is a nuclear matrix protein known to be involved in pre-mRNA splicing, ubiquitylation, and DNA repair. In human umbilical vein endothelial cells, SNEV overexpression delayed the onset of replicative senescence. Here we analyzed the function of the mouse SNEV gene in vivo by employing homologous recombination in mice and conclude that SNEV is indispensable for early mouse development. Mutant preimplantation embryos initiated blastocyst formation but died shortly thereafter. Outgrowth of SNEV-null blastocysts showed a lack of proliferation of cells of the inner cell mass, which subsequently underwent cell death. While SNEV-heterozygous mice showed no overt phenotype, heterozygous mouse embryonic fibroblast cell lines with reduced SNEV levels displayed a decreased proliferative potential in vitro. Our experiments demonstrate that the SNEV protein is essential, functionally nonredundant, and indispensable for mouse development.
Autor*innen der BOKU Wien:
Grillari Johannes
Grillari Regina
Katinger Hermann
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Blastocyst - cytology
Cell Proliferation - cytology
Crosses, Genetic - cytology
Embryo, Mammalian - pathology
Female - pathology
Fetal Death - pathology
Fibroblasts - cytology
Gene Expression Regulation - cytology
Gene Targeting - cytology
Heterozygote - cytology
Male - cytology
Mice - cytology
Mice, Inbred C57BL - cytology
NIH 3T3 Cells - cytology
Nuclear Matrix-Associated Proteins - deficiency
Nuclear Proteins - deficiency
RNA, Messenger - genetics
Sequence Analysis, Protein - genetics



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